منابع مشابه
Mucoid mutants of Escherichia coli.
The WV strain of Escherichia coli has been used extensively in analyzing various biosynthetic pathways (Davis, 1952a) and also in studying drug resistance (Davis, 1952b). This strain is nonmucoid as ordinarily cultured on solid medium at 35 C. However, among mutants selected for various properties, i. e., auxotrophy, meiotrophy4 and drug resistance, a few were observed to be mucoid. An investig...
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Homologous recombination is a fundamental biological process. Biochemical understanding of this process is most advanced for Escherichia coli. At least 25 gene products are involved in promoting genetic exchange. At present, this includes the RecA, RecBCD (exonuclease V), RecE (exonuclease VIII), RecF, RecG, RecJ, RecN, RecOR, RecQ, RecT, RuvAB, RuvC, SbcCD, and SSB proteins, as well as DNA pol...
متن کاملProduction of specific IgY antibody to the recombinant FanC protein produced in Escherichia coli
Objective(s): Enterotoxigenic Escherichia coli (ETEC) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. IgY technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. The purpose of this study was to produce specific antibody by egg yolk antibody (IgY...
متن کاملProduction of antibody fragments in Escherichia coli.
Escherichia coli is a host widely used in the industrial production of recombinant proteins. However, the expression of heterologous proteins in E. coli often encounters the formation of inclusion bodies, which are insoluble and nonfunctional protein aggregates. For the successful production of antibody fragments, which includes single-chain variable fragments (scFvs), we describe here the modi...
متن کاملRegulatory Mechanisms for Synthesis of Capsular Polysaccharide in Mucoid Mutants of Escherichia Coli K12.
Jacob and Monod have proposed that regulator genes may control the syntheses of several related enzymes in Escherichia coli K12.1 The present paper provides evidence for a regulator gene (designated R1)2 which controls capsular polysaccharide synthesis in E. coli K12. Certain mutants of E. coli produce large mucoid colonies which are characterized by overproduction of polysaccharide. The polysa...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1959
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.77.4.452-460.1959